PHENOTYPIC DETECTION OF Escherichia coli PRODUCING Extended Spectrum Beta Lactamases (ESBLs) IN THE REPRODUCTIVE TRACT BALI COW


 Escherichia coli bacteria that are resistant to β-lactam antibiotics and may be able produce Extended-spectrum β-lactamase (ESBLs) have been found in the reproductive tract of Bali cattle. ESBL will cause antimicrobial resistance (AMR) which has become a global health problem. The purpose this study was detect phenotypically presence produces from This research conducted February 2022 Lando Village, Terara District, East Lombok Regency using 8 female cattle on smallholder farms. Reproductive fluid samples were collected plastic sheet gun inserted into Brain Infusion Heart (BHI) media incubated at Public Health Calibration Laboratory West Nusa Tenggara Province. then cultured EMBA for isolation coli. isolated identified by gram staining biochemical tests based Bergey's manual determinative bacteriology. screened disc diffusion method Penicillin G10U, Ceftazidime 30 μg, Cefotaxime μg followed confirmation test double disk synergy (DDST) method. results screening showed only 12.5% (1 out samples) G, but they had not detected phenotypically.